Put the lid on the jars, but don't close the lid has to be completely loose, if not the glass jar will explode. If you are sterilizing a sample in a container (eg: agar) make sure it doesn't fill the container by more than two-thirds.
In general the pressure cooker sterilization procedure is as follows: Specific information on the latter is given in the last section of the video, as well as in this manual (which can be found online or in the Kitchen Lab). There are two types of pressure cookers in the BioFabLab: the smaller kitchen variety used for most of the video, and the larger Hawkins Big-Boy models. NOTE: Don't start the timing before the pressure have build up inside the pot, and steam starts coming out. This approach is simpler and usually faster than using the large autoclave machines in RUC's laboratories (also less scary if you're just starting off). Here is a DIY approach to sterilization of media, equipment, etc that can be done at the BioFabLab. Published: June 26, 2018, Edited by: Torben Oliver Callesen Pressure cooker (sterilization)
